Semen analysis using World Health Organization (WHO) 2010 criteria

The exact analysis of the quality of a sperm sample is crucial for couples who have difficulty in conception.

A spermodiagramm checks:

Volume Count. The volume of the semen ejaculate, when the sample collection is complete, determines indirectly the good functioning of the reproductive organs, such as his prostate and the sperm cell. The volume of the test sample should be between 1.5 and 6 ml.

Sperm Count. The number of spermatozoa produced in one ejaculation, indirectly determines the level of the testicular function of a male. According to the most recent criteria of the WHO, the number of spermatozoa should exceed 15 million spermatozoa per millilitre.

Motility of spermatozoa. Spermatozoa are characterised by:
• progressive motility
• non-progressive motility
• or they can be immotile

The total of the progressive motile spermatozoa in a fertile sperm should exceed 32% in the first hour of observation (WHO, 2010).

Morphology of spermatozoa. Morphology is also a very important parameter of the quality of spermatozoa. To analyse their morphology, sperm cells undergo a specificialized screening, using individualized observation under a  light microscope. In a fertile sample, spermatozoa with a normal morphology should exceed 5% (WHO, 2009).


• The accuracy of our sperm analysis is systematically monitored through participation in an external quality assessment system (UKNEQAS).

• In case of pathological findings, free counselling is offered on how they affect the possibility of conception and what the appropriate next steps are.

Useful tips

• To accurately determine a man’s sperm quality and fertilising capacity, at least two different analysis are necessary, with an interval of two months.
• For a semen analysis, appropriate abstinence should be 2-4 days.
• The use of drugs, recent diseases and/or recent fever can negatively affect the quality of a sperm sample. If there is any of the above-mentioned factors, it is good to delay sperm examination, so that is does not lead to false conclusions.
• Smoking, alcohol and increased body weight are factors negatively affected sperm quality.